Device

Part:BBa_K1015016

Designed by: Kento Tominaga   Group: iGEM13_TMU-Tokyo   (2013-09-16)

lacI+FRT+Cm

This is genome insertion part.

The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009).


Results of aagarose gel electrophoresis

TMU-Tokyo_0904.jpg
Figure. The left is molecular weight marker which is λ-hindⅢand the right is the device(K1015013).
In this result, its DNA band is appeared deeply.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1447
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1447
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None